SEQSERVICE

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(New page: The sequences database has been developed by CEINGE bioinformatics group and offers integrated support for CAPRI, our web interface for useful tools devoted to editing and analysis of nucl...)
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-The sequences database has been developed by CEINGE bioinformatics group and offers integrated support for CAPRI, our web interface for useful tools devoted to editing and analysis of nucleic and protein sequences.+The production of large amount of nucleotide sequences is used in biology, genetics and medicine, for example, in order to sequence genomic regions, or to identify novel mutations, or to identify genes selected in gene expression and protein interaction studies. In such applications the availability of a system able not only to collect and manage the sequences, but also to analyze the results with modern bioinformatic techniques, may be very useful.
-The CEINGE DNA Sequencing Core provides investigators access to high-quality automated DNA Sequencing technology. Sequences are performed by the ET-terminators kit (Amersham Biosciences) and run on the MegaBACE 500 (Amersham Biosciences) capillary sequencer. +For this reason we realized a web-based sequences database able to help the researcher in all phases of the sequencing process, from sample submission, to sample processing, to results retrieving and analysis. This system works around the CEINGE sequencing service and, in fact, it also represents an interface between researchers and service operators.
-Turnaround time is very short; results are usually available within 48-72 hours of sample submission. All phases of the sequencing process, from sample submission, to sample processing, to results retrieving are highly automated and work around a web-based sequences database. + 
-To access to CEINGE bioinformatic services it is necessary to have an account.+The sequences, assigned to a specific project, can be controlled at any stage of their processing and collected in the database. Each sample includes additional information such as primer sequence, template type and hypothetical amplimer size. The system includes advanced analysis routines which can be used for sets of sequences of variable size: 1) filtering of repeated regions and/or vector sequences; 2) sequence identification by searching homologies in different datasets; 3) ‘contigs’ assembly by using the PHRED/PHRAP/CONSED package. More sophisticated analysis are provided by the integrated support for [[CAPRI]], our solution for editing and analyzing nucleic and protein sequences.
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 +To access to the system, currently limited to registered users, please click [http://sequences.ceinge.unina.it here].
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 +[[seqservice:userinfo|User info]]
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 +{{footer|footername=footer facilities}}

Current revision

The production of large amount of nucleotide sequences is used in biology, genetics and medicine, for example, in order to sequence genomic regions, or to identify novel mutations, or to identify genes selected in gene expression and protein interaction studies. In such applications the availability of a system able not only to collect and manage the sequences, but also to analyze the results with modern bioinformatic techniques, may be very useful. For this reason we realized a web-based sequences database able to help the researcher in all phases of the sequencing process, from sample submission, to sample processing, to results retrieving and analysis. This system works around the CEINGE sequencing service and, in fact, it also represents an interface between researchers and service operators.

The sequences, assigned to a specific project, can be controlled at any stage of their processing and collected in the database. Each sample includes additional information such as primer sequence, template type and hypothetical amplimer size. The system includes advanced analysis routines which can be used for sets of sequences of variable size: 1) filtering of repeated regions and/or vector sequences; 2) sequence identification by searching homologies in different datasets; 3) ‘contigs’ assembly by using the PHRED/PHRAP/CONSED package. More sophisticated analysis are provided by the integrated support for CAPRI, our solution for editing and analyzing nucleic and protein sequences.

To access to the system, currently limited to registered users, please click here.


User info


Bioinformatics: CEINGE facilities: SEQSERVICE - OLIGOS - CELLBANK
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